Published on Friday, 12 March 2021
Abstract
The present study aimed to evaluate the effect of melatonin on DNA Double-Strand Breaks repair using gene expression change of Ku70 and Xrcc4 in rat peripheral blood.
One hundred eight male rats were randomly divided in six different groups of control, vehicle-only, melatonin alone, irradiation-only, vehicle plus irradiation, and melatonin plus irradiation.
Rats were given an intraperitoneal (IP) injection of melatonin (100 mg/kg) 1 hr prior to irradiation. Irradiation was done with 2Gy whole-body radiation by linear accelerator.
Peripheral blood samples were collected at 8, 24 and 48 h after irradiation and then RNA was immediately extracted from the peripheral blood lymphocytes and the mRNA transcriptional changes of Ku70 and XRCC4 were evaluated by real-time quantitative polymerase chain reaction (Real-time PCR).
Irradiation-only group showed an increase in gene expression change of Ku70 and XRCC4, which was significantly different from control group (P<0.01) in 24 h post-irradiation, while in melatonin plus irradiation group, Ku70 and XRCC4 genes were upregulated significantly compared to control group (P<0.01) at all three post-irradiation times.
It is concluded that melatonin may provide modulation of Ku70 and XRCC4 expression to protect rat peripheral blood lymphocytes against ionizing radiation.
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See also:
- Official Web Site: The Di Bella Method;
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- Melatonin with adenosine solubilized in water and stabilized with glycine for oncological treatment - technical preparation, effectivity and clinical findings;
- About Melatonin - In vitro, review and in vivo publications;
- Publication: Melatonin anticancer effects: Review (from Di Bella's Foundation);
- Publication: Key aspects of melatonin physiology: 30 years of research (from Di Bella's Foundation);
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- Complete objective response to biological therapy of plurifocal breast carcinoma;
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